Metabolites during plant mitosis are clarified

March 18, 2022

Single cell analysis captured the contents of cells with high accuracy

A research team of the RIKEN CSRS and the Center for Biosystems Dynamics Research revealed the variation of metabolites in plant cells during the mitotic sub-phase (prophase, metaphase, anaphase, and telophase) at the single-cell level.

It has been expected that understanding the metabolite profile in cells during mitosis would help reveal the essential factors behind the process of mitosis and the insertion mechanism of cell plates. However, the composition of metabolites in cells during each mitotic sub-phase has remained unknown.

The research team established the technique for the single-cell metabolome analysis for plant mitosis using a tobacco cell line with visualized chromosomes and successfully figured out the variation of cellular metabolites during mitosis.

The findings of this research will contribute to understanding the mitotic mechanism of plant cells accompanied by the insertion of “cell plates” and further developing technology applicable to plant biomass.

Original article

Plant Physiology doi:10.1093/plphys/kiac093

E. Okubo-Kurihara, A. Ali, M. Hiramoto, Y. Kurihara, Y. Abouleila, E. Abdelazem, T. Kawai, Y. Makita, M. Kawashima, T. Esaki, H. Shimada, T. Mori, M. Hirai, T. Higaki, S. Hasezawa, Y. Shimizu, T. Masujima, M. Matsui,

"Tracking metabolites at single cell-resolution reveals metabolic dynamics during plant mitosis".

Contact

Emiko Kurihara; Research Scientist

Minami Matsui; Group Director

Synthetic Genomics Research Group