Measuring stiffness of cells with DNA sequencing
May 17, 2024
Contributing to the understanding of the mechanism to control cellular mechanic properties
A joint research group of the RIKEN Cluster for Pioneering Research, the RIKEN CSRS, Toyohashi University of Technology, and the Department of Hematology at the University of Tsukuba has developed a technology to assess a single cell’s mechanical property (cell surface tension) and gene expression simultaneously on a large scale.
The research group combined electroporation, which introduces molecules into cells, and single-cell RNA sequencing to develop a technology to assess the mechanical properties and gene expression of a single cell concurrently on a large scale, named “ELASTomics.” Then, using human TIG-1 cells, the researchers showed that RRAD could be a factor in inducing the increase of cell surface tension in senescent cells.
The findings of this study are expected to contribute to the further understanding of gene regulation mechanisms that underlie various biological phenomena and diseases involving cellular mechanical properties, such as aging, cellular differentiation, and cancer cell infiltration.
- Original article
- Nature Communications doi: 10.1038/s41467-024-48088-5
- A. Shiomi, T. Kaneko, K. Nishikawa, A. Tsuchida, T. Isoshima, M. Sato, K. Toyooka, K. Doi, H. Nishikii, H. Shintaku,
- "High-throughput mechanical phenotyping and transcriptomics of single cells".
- Contact
- Kiminori Toyooka
Senior Technical Scientist
Mass Spectrometry and Microscopy Unit
