Charles M. Boone
- Ph.D., McGill University, Canada
- Assistant Professor, Simon Fraser University, Canada
- Assistant Professor, Queen's University, Canada
- Associate Professor, University of Toronto, Canada
- Professor, University of Toronto, Canada
- Senior Visiting Scientist, RIKEN
- Team Leader, Molecular Ligand Target Research Team, RIKEN
- Team Leader, Molecular Ligand Target Research Team, RIKEN Center for Sustainable Resource Science (-current)
Small molecular ligands with unique activities must have the specific target molecules that exist in their cells or organisms. Identification of the target molecules is critical for elucidating the mode of action of the molecular ligands and for drug development. However, drug target identification has been difficult in general, because the mode of interactions between the molecular ligands and their targets is not uniform. Our team aims at developing innovative techniques based on global analysis of yeast genetic interaction, which leads to quick and accurate detection of the ligand-target interactions.
- Global analysis of genetic interaction between molecular ligands and their target molecules
- Mode of action of bioactive compounds
- A microarray-based method for identification of drug targets using the fission yeast ORFeome
- We created a novel strain collection in a drug-sensitive genetic background that expresses the entire fission yeast ORFeome. We can now pool this library and grow the yeast in the presence of a drug. The abundance of each strain is assessed using DNA microarray to generate a genome-wide profile of drug-gene interactions. This chemical genomics approach can rapidly determine genes involved in pathways targeted by small molecules.