Real-time visualization of plant epigenetic dynamics
April 18, 2017
A fragment of the mouse antibody recognizes an antigen in a living plant cell
A joint research group led by researchers from Tokyo University of Science, RIKEN CSRS and the Tokyo Institute of Technology has developed a method for live-cell imaging of plant epigenetic changes by expression of a fragment of the mouse antibody.
Researchers created the transgenic tobacco cultured cells, which expresses the mintbody (modification-specific intracellular antibody, a fluorescent protein fused to an antibody fragment). The mintbody recognized an acetylated lysine residue, which is a type of histone modification. Livecell imaging, inhibitor experiments and biochemical experiments revealed that the mintbody correctly recognizes histone acetylation on lysine residues. This is the first report of a mousederived mintbody working in a plant cell. Plants do not have intracellular antibodies. Because the antibody fragment was produced in the plant cells and recognized the plant histone modification, our results could lead to develop a new functional tool for plant research.
These results make it possible to analyze epigenetic changes in plants over a time course and should contribute to progress in understanding epigenetic-controlled plant environmental responses and elucidating environmental memory mechanisms. The ability to express an animal antibody fragment in a plant cell and conduct biochemical and cell biology research should also greatly contribute to plant science and agricultural research.
- Original article
- Scientific Reports doi:10.1038/srep45894
- K. Kurita, T. Sakamoto, N. Yagi, Y. Sakamoto, A. Ito, N. Nishino, K. Sako, M. Yoshida, H. Kimura, M. Seki, S. Matsunaga,
- "Live imaging of H3K9 acetylation in plant cells".
- Motoaki Seki; Team Leader, Plant Genomic Network Research Team
- Minoru Yoshida; Group Director, Chemical Genomics Research Group