Palladium complex captures β-ketoamide-tagged peptides

July 25, 2023

Applying metal catalysts to chemical biology research

The research team, consisting of members from the RIKEN Cluster for Pioneering Research and the RIKEN CSRS, has successfully developed an affinity purification method that captures β-ketoamide-tagged peptides from a mixture of peptides by applying the palladium (Pd) complex created as a catalyst for asymmetric reactions to chemical biology research.

The research team developed various asymmetric reactions using Pd complexes as catalysts. On the way, it was found that a Pd complex forms a stable complex with a reactive substrate, β-ketoamide (R-CO-CH2-CO-NH-R’). In this study, the team developed a method to capture peptides tagged with a compact functional group, β-ketoamide, from a peptide mixture by using a solid-supported Pd complex. Using this method, the researchers successfully identified the binding site of the enzyme inhibitor to its target protein.

The findings of this research are expected to contribute to analyzing chemical protein modificatioins and action mechanisms of bioactive compounds.

Original article
Chemical Science doi: 10.1039/d2sc03112d
K. Hayamizu, K. Koike, Kosuke Dodo, M. Asanuma, H. Egami, M. Sodeoka,
"Simple Purification of Small-Molecule-Labelled Peptides via Palladium Enolate Formation from β-Ketoamide Tags".
Kosuke Dodo; Senior Research Scientist
Mikiko Sodeoka; Group Director
Catalysis and Integrated Research Group