Development of “MS-DIAL 5”, a program for analyzing complex and diverse lipid metabolism information

November 29, 2024

Enabling understanding of lipid structures and localization from multimodal mass spectrometry data

An international collaborative research group consisting of researchers from Tokyo University of Agriculture and Technology, Keio University, RIKEN Center for Integrative Medical Sciences, and RIKEN CSRS has developed "MS-DIAL 5," a mass spectrometry data analysis program designed for lipid diversity profiling, identification of lipid isomer structures, and visualization of lipid localization.

Using MS-DIAL 5, the group analyzed the lipid diversity and localization in mouse eyes, revealing part of the molecular mechanism by which very-long-chain polyunsaturated fatty acids (VLC-PUFAs), considered critical for maintaining visual function, are incorporated into specific sites within phosphatidylcholine, a type of phospholipid that forms cell membranes.

In the current era, where various mass spectrometry methods are available for capturing lipid metabolism, the lack of informatics programs capable of processing and integrating diverse (multimodal) data has been a bottleneck. MS-DIAL 5, which enables comprehensive analysis of such data, is expected to serve as a transformative tool to accelerate both basic and applied research in life sciences.

Original article

Nature Communications doi: 10.1038/s41467-024-54137-w

H. Takeda, Y. Matsuzawa, M. Takeuchi, M. Takahashi, K. Nishida, T. Harayama, Y. Todoroki, K. Shimizu, N. Sakamoto, T. Oka, M. Maekawa, M. H. Chung, Y. Kurizaki, S. Kiuchi, K. Tokiyoshi, B. Buyantogtokh, M. Kurata, A. Kvasnička, U. Takeda, H. Uchino, M. Hasegawa, J. Miyamoto, K. Tanabe, S. Takeda, H. Tsugawa,

"MS-DIAL 5 multimodal mass spectrometry data mining unveils lipidome complexities".

Contact

Hiroshi Tsugawa
Visiting Scientist
Metabolome Informatics Research Team