Joint Research Promotion Program (JRPP) in Wako

In order to promote joint research (especially within CSRS), we will present the advanced technologies of the "Advanced Research and Technology Platforms" and consult on joint research.

This is a service for CSRS. Please contact us if you are outside.

Outline of the Advanced Research and Technology Platforms link to Molecular interactions link to High throughput screening link to Proteins and supermolecules link to Chemicals and small molecules link to Persons in charge

Chemicals and small molecules

  • Diverse chemical library provision for High Throughput Screening (HTS) (Mr Hirano)
  • Provision of chemical libraries for efficient compound screening (Mr Hirano)
    1. Authentic library (80 Highly active compounds)
    2. Pilot library (Typical 400 chemotype compounds)
    3. Bioactive focused chemical Libraries
    Anti-fungal action, Anti-parasite, Antiviral effect, Bacterial growth, Bacterial growth, Cell morphology change, Cell proliferation, Effects on plants, Enzyme inhibition, Kinase inhibition, Protein-protein interactions,
  • Support of Information Search related to hit compounds (Mr Hirano)
  • (published literature, novelty, purchase potential)
  • Support of compound similarity search for analogs of hit compounds and provision of these compounds (Mr Hirano)
  • (Comparison among a large number of compound groups, a number of compounds structure search for the NPDepo library and selection of duplicate compounds)
  • Structural classification for the hit compounds set by hierarchical clustering method (Mr Hirano)
  • 2DE-based proteomic analysis of proteins changed in thermal stability by binding to a compound (2DE-CETSA) (Dr Muroi)
  • Making chemical beads for target identification (Dr Kondoh)
  • Synthesis of bioactive compounds (Dr Hagihara)
  • Chemical tools that accelerate plant science (Dr Hagihara)
  • Mass spectrometry in the qualitative and quantitative analysis of organic compounds (Dr Muranaka)
  • (including but not limited to: accurate mass measurement and deduction of molecular mass and formula, analysis of unstable compounds including air-sensitive ones, analysis of volatile compounds including volatile derivatives, method development for qualitative and/or quantitative mass spectrometry, structural characterization based on tandem mass spectrometry and ion mobility spectrometry, analysis and characterization of isomers)
  • Measurement and analysis of NMR spectra (Dr Muranaka)
  • (structural characterization of organic compounds using NMR spectroscopy, measurement of several NMR experiments,quantitative NMR analysis, measurement and analysis of solid-state NMR spectra, measurement of micro magnetic resonance imaging)
  • Measurement and analysis of circular dichroism spectra (Dr Muranaka)
  • Measurement and analysis of UV-vis-NIR, IR, and fluorescence spectra (Dr Muranaka)
  • Measurement of cyclic voltammograms (Dr Muranaka)
  • Molecular structure analysis based on quantum chemical calculations (Dr Muranaka)
  • Determination of absolute configuration of organic compounds based on organic synthesis and asymmetric total synthesis (Dr Muranaka)

High-throughput screening

  • Target identification of small molecules and functional genomics with shRNA screening and CRISPR screening in human cells (Dr Matsumoto)
  • Assay development for High-throughput screening (HTS) and High-content screening (HCS) (Dr Idei)
  • Target identification based on yeast and human chemical genomics analysis (Dr Yashiroda)

Proteins and supermolecules

  • Quantitative Proteomics (Dr Dohmae)
  • Protein post-translational modifications analysis (Dr Dohmae)
  • Identification of binding site of the compounds in protein (Dr Dohmae)
  • Characterization of RNA and therapeutic oligonucleotides using MS (Dr Dohmae)
  • Protein absolute quantification using amino acid analysis (Dr Dohmae)
  • Cell free synthesis of protein and peptide (E. coli, Wheat Germ and human) (Dr Dohmae)
  • Mass spectrometry of compounds and macromolecules including determination of exact mass (Dr Dohmae)
  • Purification and analysis using HPLC (Dr Dohmae)
  • X-ray crystallography (Dr Kudo)
  • Protein knockdown by AID system (Dr Hagihara)

Molecular interactions

  • Interaction analysis using NanoBiT (Split Luc) (Dr Sasaki)
  • Binding affinity measurement of biomolecular interactions by Microscale Thermophoresis (MST) (Mr Matsuoka)
  • Kinetics analysis of biomolecular interactions by Surface Plasmon Resonance (SPR) (Mr Matsuoka)
  • Thermodynamic analysis of biomolecular interactions by Isothermal Titration Calorimetry (ITC) (Mr Matsuoka)
  • Evaluation of protein stability by Nano Differential Scanning Fluorimetry (nanoDSF) (Mr Matsuoka)

Equipments

2DE-based proteome analysis system (Dr Muroi)
2DE-based proteome analysis system photo

High Throughput DNA Purification instrument (Dr Yashiroda)
High Throughput DNA Purification instrument photo

Quantitative Proteomics・ Protein post-translational modifications analysis (Dr Dohmae)
Quantitative Proteomics・ Protein post-translational modifications analysis photo  Quantitative Proteomics・ Protein post-translational modifications analysis photo
Quantitative Proteomics・ Protein post-translational modifications analysis photo  Quantitative Proteomics・ Protein post-translational modifications analysis photo

Protein absolute quantification using amino acid analysis (Dr Dohmae)
Protein absolute quantification using amino acid analysis photo

Purification and analysis using HPLC (Dr Dohmae)
Purification and analysis using HPLC photo

Stereomicroscope and calculator (Dr Kudo)
Stereomicroscope and calculator photo

Persons in charge

Dr Ken Matsumoto ( Chemical Genomics Research Group )
Dr Kazuki Sasaki ( Chemical Genomics Research Group )
Dr Norio Kudo ( Chemical Genomics Research Group )
Dr Yoko Yashiroda ( Molecular Ligand Target Research Team )
Dr Yasumitsu Kondoh( Chemical Biology Research Group )
Dr Makoto Muroi ( Chemical Biology Research Group )
Mr Seiji Matsuoka( Drug Discovery Seed Compounds Exploratory Unit | Drug Discovery Platforms Cooperation Division )
Dr Akiko Idei ( Drug Discovery Seed Compounds Exploratory Unit | Drug Discovery Platforms Cooperation Division )
Mr Hiroyuki Hirano ( Drug Discovery Chemical Bank Unit | Drug Discovery Platforms Cooperation Division )
Dr Shinya Hagihara ( Molecular Bioregulation Research Team )
Dr Atsuya Muranaka ( Molecular Structure Characterization Unit | Technology Platform Division )
Dr Naoshi Dohmae ( Biomolecular Characterization Unit | Technology Platform Division )
 

Contact us

It is in trial operation. Please contact us by email at CSRScollabo [at] ml.riken.jp
We will contact you for a meeting on Zoom.